Pentanucleotide TTTTA and G/A -914 DNA polymorphisms in apolipoprotein(a) promoter: genotyping by single-tube PCR

Clin Chem Lab Med. 2003 May;41(5):632-3. doi: 10.1515/CCLM.2003.095.

Abstract

It is known that pentanucleotide repeat polymorphism, consisting of varying number of (TTTTA) repeats in a promoter, is associated with plasma lipoprotein(a) levels. G/A -914 base substitution has also been correlated with pentanucleotide repeat polymorphism in individuals with high and low lipoprotein(a) levels. To facilitate future analysis of these polymorphisms we have developed a quick and reliable assay for the detection of both polymorphisms in a single polymerase chain reaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apolipoproteins / blood*
  • Apolipoproteins / genetics*
  • Apoprotein(a)
  • DNA / blood*
  • DNA / genetics*
  • Genotype
  • Humans
  • Lipoprotein(a) / blood*
  • Lipoprotein(a) / genetics*
  • Microsatellite Repeats*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*

Substances

  • Apolipoproteins
  • Lipoprotein(a)
  • DNA
  • Apoprotein(a)