The genetic structure of eight populations of four locust species in three families (Catantopidae, Pamphagidae and Pyrgomorphidae) was examined by means of horizontal starch gel electrophoresis, the locusts were collected from Shanxi, Jiangsu and Hebei Province in China. The allele frequency and allozyme polymorphism of 12 enzymes (18 loci) were analyzed. Low variability with a few alleles was observed in Shirakiacris shirakii and Atractomorpha sinensis, however, Oxya Chinensis and Haplotropis brunneriana showed high polymorphism. In the eight populations of the four locust species, high percentage of polymorphic loci was observed (53.3%-100.0%). Owing to heterozygote deficiency of some species, all the four species demonstrated low overall heterozygosity (Ho = 0.034-0.139), which lead to the genotype frequency deviated significantly from Hardy-Weinberg equilibrium. However, many of the examined loci were fit for or close to H-W equilibrium in Atractomorpha sinensis. A certain differentiation in mean heterozygosity was found among the four species. Due to the difference of migratory capability, reproductive manner and living bound, mean heterozygosity (Ho) is higher in Haplotropis brunneriana (Ho = 0.089-0.139) and Oxya Chinensis (Ho = 0.073-0.090) than in Atractomorpha sinensis (Ho = 0.034-0.050) and Shirakiacris shirakii (Ho = 0.048-0.068). Genetic differentiation from F-statistics was also different at population level among four locust species. It was high in Haplotropis brunneriana (Fst = 0.32) and Atractomorpha sinensis (Fst = 0.31), and low genetic differentiations were observed in Oxya Chinensis (Fst = 0.20) and Shirakiacris shirakii (Fst = 0.18). It was confirmed that migratory capability, adaptability and surrounding factors had an important influence on the genetic differentiation of locust populations. The taxon relationships based on Nei's genetic identity (I) and genetic distance (D) were consistent with results obtained from karyotypic analyses. Oxya Chinensis and Shirakiacris shirakii in the same family have a higher genetic identity (I = 0.576) and a smaller genetic distance (D = 0.559); the species examined in Pampagidae displayed somewhat closer relationship to those in Pyrgomorphidae (D = 0.776, I = 0.464) than to those in Catantopidae (D = 0.908, I = 0.406). It is suggested that the allozyme analysis is an useful molecular marker for phylogenetic reconstruction of locust at both species and population levels.