Ruthenium complexes carrying a disialo complex-type oligosaccharide: enzymatic synthesis and its application to a luminescent probe to detect influenza viruses

Shinji Kojima; Teruaki Hasegawa; Takahiro Yonemura; Ken Sasaki; Kenji Yamamoto; Yutaka Makimura; Tadanobu Takahashi; Takashi Suzuki; Yasuo Suzuki; Kazukiyo Kobayashi

doi:10.1039/b210739b

Ruthenium complexes carrying a disialo complex-type oligosaccharide: enzymatic synthesis and its application to a luminescent probe to detect influenza viruses

Chem Commun (Camb). 2003 Jun 7:(11):1250-1. doi: 10.1039/b210739b.

Authors

Shinji Kojima¹, Teruaki Hasegawa, Takahiro Yonemura, Ken Sasaki, Kenji Yamamoto, Yutaka Makimura, Tadanobu Takahashi, Takashi Suzuki, Yasuo Suzuki, Kazukiyo Kobayashi

Affiliation

¹ Department of Molecular Design, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.

PMID: 12809215
DOI: 10.1039/b210739b

Abstract

Tris-bipyridine ruthenium-complexes carrying a disialo complex-type oligosaccharide were prepared via a one-pot transglycosylation using endo-glycosidase (Endo M); they bind to type-A influenza viruses with excellent affinity (IC50 = 8.4 microM), and their luminescence intensity is strongly depressed by virus-binding.

MeSH terms

Enzymes / chemistry*
Luminescence
Molecular Probes*
Oligosaccharides / chemistry*
Orthomyxoviridae / isolation & purification*
Ruthenium / chemistry*

Substances

Enzymes
Molecular Probes
Oligosaccharides
Ruthenium