Detection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03

FEMS Microbiol Lett. 2003 Jun 6;223(1):47-51. doi: 10.1016/S0378-1097(03)00329-X.

Abstract

The Bacillus subtilis strain KS03 was isolated, and identified as a biological control agent that inhibits the anthracnose disease fungus Gloeosporium gloeosporioides. The antifungal compound was purified from its culture broth through butanol extraction, diethylaminoethyl (DEAE) Sepharose CL-6B chromatography, and preparative thin layer chromatography. Tandem mass spectrometric analyses (MS/MS), with matrix-assisted laser desorption ionization (MALDI) time-of-fight/time-of-flight (TOF/TOF) mass spectrometry, showed that the antifungal compound was iturin A, a cyclic lipopeptide antibiotic. The major compound, with a molecular mass of 1042 Da, was identified as iturin A(2).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / analysis
  • Anti-Bacterial Agents / pharmacology*
  • Antifungal Agents / analysis
  • Antifungal Agents / pharmacology*
  • Bacillus subtilis / chemistry*
  • Bacillus subtilis / genetics
  • Mitosporic Fungi / drug effects*
  • Mitosporic Fungi / growth & development
  • Peptides*
  • Peptides, Cyclic
  • RNA, Ribosomal, 16S / genetics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Anti-Bacterial Agents
  • Antifungal Agents
  • Peptides
  • Peptides, Cyclic
  • RNA, Ribosomal, 16S
  • iturin A