Abstract
The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-rexA+-rexB+ derivatives. (ii) The cI[Ts]857 allele augmentation of Rex exclusion is recessive to cI+. (iii) The cI857-mediated increase in Rex exclusion activity involves the participation of a genetic element mapping outside of cI-rexA-rexB.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Bacteriophage lambda / genetics*
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DNA-Binding Proteins*
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Escherichia coli / genetics
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Genes, Viral*
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Models, Genetic
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Phenotype
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Plasmids / analysis
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Viral Nonstructural Proteins / genetics*
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Viral Proteins / genetics*
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Viral Regulatory and Accessory Proteins
Substances
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DNA-Binding Proteins
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Repressor Proteins
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RexA protein, Bacteriophage lambda
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Viral Nonstructural Proteins
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Viral Proteins
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Viral Regulatory and Accessory Proteins
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phage repressor proteins
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rexB protein, Bacteriophage lambda