N-Glycan structures of squid rhodopsin

Eur J Biochem. 2003 Jun;270(12):2627-32. doi: 10.1046/j.1432-1033.2003.03636.x.

Abstract

To determine the glycoforms of squid rhodopsin, N-glycans were released by glycoamidase A digestion, reductively aminated with 2-aminopyridine, and then subjected to 2D HPLC analysis [Takahashi, N., Nakagawa, H., Fujikawa, K., Kawamura, Y. & Tomiya, N. (1995) Anal. Biochem.226, 139-146]. The major glycans of squid rhodopsin were shown to possess the alpha1-3 and alpha1-6 difucosylated innermost GlcNAc residue found in glycoproteins produced by insects and helminths. By combined use of 2D HPLC, electrospray ionization-mass spectrometry and permethylation and gas chromatography-electron ionization mass spectrometry analyses, it was revealed that most (85%) of the N-glycans exhibit the novel structure Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Galbeta1-4Fucalpha1-6)(Fucalpha1-3)GlcNAc.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbohydrate Sequence
  • Decapodiformes / chemistry*
  • Glycoproteins / chemistry*
  • Molecular Sequence Data
  • Mollusca / chemistry*
  • Oligosaccharides / chemistry*
  • Polysaccharides / chemistry*
  • Rhodopsin / chemistry*
  • Spectrometry, Mass, Electrospray Ionization
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Glycoproteins
  • Oligosaccharides
  • Polysaccharides
  • Rhodopsin