Cloning the full-length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function

Chin Med J (Engl). 2003 Feb;116(2):166-70.

Abstract

Objective: To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell (v-SMC) proliferation in atherogenesis, and study its function.

Methods: Oxidized low density lipoprotein (ox-LDL) at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium. A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique. Methods, including blotting, Northern hybridization and gene sequencing, were used to clone new gene fragments. By using full-length cDNA screening and protein expression techniques, one full-length cDNA was cloned and its function was studied.

Results: One full-length cDNA was cloned. The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox-LDL.

Conclusion: The new gene cloned may be associated with SMC proliferation in atherogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arteriosclerosis / genetics*
  • Base Sequence
  • Blotting, Northern
  • Cell Division / drug effects
  • Cells, Cultured
  • Cloning, Molecular
  • Gene Library
  • Humans
  • Lipoproteins, LDL / pharmacology*
  • Molecular Sequence Data
  • Muscle, Smooth, Vascular / cytology*
  • Muscle, Smooth, Vascular / drug effects*
  • Nucleic Acid Hybridization

Substances

  • Lipoproteins, LDL
  • oxidized low density lipoprotein

Associated data

  • GENBANK/AF174647