Abstract
Restriction fragment length polymorphism (RFLP) is used to differentiate microorganisms by analysis of their DNA restriction patterns. A modified RFLP procedure is proposed for the rapid characterization of Lactococcus lactis strains producing exopolysaccharides (EPS). The availability of such effective cataloging system is likely to benefit research aimed at identifying lactococcal strains that produce novel EPS.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Bacterial / analysis
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DNA, Bacterial / metabolism
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Lactococcus lactis / classification*
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Lactococcus lactis / genetics
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Lactococcus lactis / metabolism*
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Polymorphism, Restriction Fragment Length*
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Polysaccharides / biosynthesis*
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Polysaccharides / genetics
Substances
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DNA, Bacterial
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Polysaccharides
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endodeoxyribonuclease SacI
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Deoxyribonucleases, Type II Site-Specific
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GRCGYC-specific type II deoxyribonucleases
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GTYRAC-specific type II deoxyribonucleases