Abstract
The proliferation of normal human fibroblast cells was enhanced by the addition of inorganic polyphosphate (poly(P)) into culture media. The mitogenic activities of acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) were also enhanced by poly(P). A physical interaction between poly(P) and FGF-2 was observed, and FGF-2 was both physically and functionally stabilized by poly(P). Furthermore, poly(P) facilitated the FGF-2 binding to its cell surface receptors. Because poly(P) is widely distributed in mammalian tissues, it may be a spontaneous modulator of FGFs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Cell Division / drug effects
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Cells, Cultured
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Culture Media
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Fibroblast Growth Factor 1 / metabolism
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Fibroblast Growth Factor 1 / pharmacology
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Fibroblast Growth Factor 2 / metabolism
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Fibroblast Growth Factor 2 / pharmacology
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Fibroblast Growth Factors / metabolism
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Fibroblast Growth Factors / pharmacology*
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Humans
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Kinetics
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Mice
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Mitogens / metabolism
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Mitogens / pharmacology*
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Polyphosphates / metabolism
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Polyphosphates / pharmacology*
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Protein Binding
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Receptor Protein-Tyrosine Kinases / metabolism
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Receptor, Fibroblast Growth Factor, Type 2
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Receptors, Fibroblast Growth Factor / metabolism
Substances
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Culture Media
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Mitogens
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Polyphosphates
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Receptors, Fibroblast Growth Factor
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Fibroblast Growth Factor 2
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Fibroblast Growth Factor 1
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Fibroblast Growth Factors
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FGFR2 protein, human
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Fgfr2 protein, mouse
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Receptor Protein-Tyrosine Kinases
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Receptor, Fibroblast Growth Factor, Type 2