cDNA microarray analysis reveals chop-10 plays a key role in Sertoli cell injury induced by bisphenol A

Biochem Biophys Res Commun. 2003 May 23;305(1):54-61. doi: 10.1016/s0006-291x(03)00708-3.

Abstract

We examined the time course of changes in gene expression in detail using cDNA microarray analysis of mouse Sertoli TTE3 cells treated with bisphenol A (BPA). A subtoxic dose of BPA (200 microM) transiently increased intracellular Ca(2+) concentration and time-dependently induced an increase in mRNA level of 78-kDa glucose-regulated protein, indicating that BPA induces endoplasmic reticulum stress. Of the 865 genes analyzed, 31 genes showed increased levels of expression. TaqMan analysis confirmed that the mRNA levels of chop-10, fra-2, c-myc, and ornithine decarboxylase were increased, and showed that chop-10 is the most sensitive gene. The expression level of chop-10 protein and cell injury induced by BPA were significantly reduced in stable TTE3 cells overexpressing full-length chop-10 antisense RNA. We conclude that chop-10 plays a key role in Sertoli cell injury induced by BPA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzhydryl Compounds
  • CCAAT-Enhancer-Binding Proteins / physiology*
  • Cell Line
  • Endoplasmic Reticulum / drug effects
  • Estrogens, Non-Steroidal / toxicity*
  • Gene Expression Profiling
  • Kinetics
  • Male
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Phenols / toxicity*
  • RNA, Messenger / biosynthesis
  • Sertoli Cells / drug effects*
  • Sertoli Cells / metabolism
  • Transcription Factor CHOP
  • Transcription Factors / physiology*
  • Up-Regulation

Substances

  • Benzhydryl Compounds
  • CCAAT-Enhancer-Binding Proteins
  • Ddit3 protein, mouse
  • Estrogens, Non-Steroidal
  • Phenols
  • RNA, Messenger
  • Transcription Factors
  • Transcription Factor CHOP
  • bisphenol A