Coupling capillary electrochromatography with electrospray Fourier transform mass spectrometry for characterizing complex oligosaccharide pools

Anal Chem. 2003 Apr 1;75(7):1684-90. doi: 10.1021/ac025985c.

Abstract

To deal with the complexity of the glycan mixtures released from glycoproteins, an efficient form of micro-column separations, capillary electrochromatography, has been combined with high-performance mass spectrometry (Fourier transform ion cyclotron resonance). Contour plots have been generated from the mixtures of O-linked oligosaccharides originated from bovine mucin and bile salt-stimulated lipase, a large glycoprotein enzyme.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Chromatography / instrumentation
  • Chromatography / methods*
  • Electrophoresis, Capillary / instrumentation
  • Electrophoresis, Capillary / methods*
  • Fourier Analysis
  • Glycoproteins / chemistry*
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods*
  • Mucins / chemistry
  • Oligosaccharides / analysis*
  • Oligosaccharides / chemistry*
  • Sterol Esterase / chemistry

Substances

  • Glycoproteins
  • Mucins
  • Oligosaccharides
  • bile salt-stimulated lipase
  • Sterol Esterase