Abstract
To deal with the complexity of the glycan mixtures released from glycoproteins, an efficient form of micro-column separations, capillary electrochromatography, has been combined with high-performance mass spectrometry (Fourier transform ion cyclotron resonance). Contour plots have been generated from the mixtures of O-linked oligosaccharides originated from bovine mucin and bile salt-stimulated lipase, a large glycoprotein enzyme.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cattle
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Chromatography / instrumentation
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Chromatography / methods*
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Electrophoresis, Capillary / instrumentation
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Electrophoresis, Capillary / methods*
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Fourier Analysis
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Glycoproteins / chemistry*
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Mass Spectrometry / instrumentation
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Mass Spectrometry / methods*
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Mucins / chemistry
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Oligosaccharides / analysis*
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Oligosaccharides / chemistry*
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Sterol Esterase / chemistry
Substances
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Glycoproteins
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Mucins
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Oligosaccharides
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bile salt-stimulated lipase
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Sterol Esterase