DAF-16 target genes that control C. elegans life-span and metabolism

Science. 2003 Apr 25;300(5619):644-7. doi: 10.1126/science.1083614. Epub 2003 Apr 10.

Abstract

Signaling from the DAF-2/insulin receptor to the DAF-16/FOXO transcription factor controls longevity, metabolism, and development in disparate phyla. To identify genes that mediate the conserved biological outputs of daf-2/insulin-like signaling, we used comparative genomics to identify 17 orthologous genes from Caenorhabditis and Drosophila, each of which bears a DAF-16 binding site in the promoter region. One-third of these DAF-16 downstream candidate genes were regulated by daf-2/insulin-like signaling in C. elegans, and RNA interference inactivation of the candidates showed that many of these genes mediate distinct aspects of daf-16 function, including longevity, metabolism, and development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Binding Sites
  • Caenorhabditis / genetics
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / physiology
  • Computational Biology
  • Conserved Sequence
  • Down-Regulation
  • Drosophila / genetics
  • Forkhead Transcription Factors
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genes, Helminth*
  • Genes, Insect
  • Genomics
  • Insulin / metabolism
  • Longevity / genetics
  • Mutation
  • Promoter Regions, Genetic
  • RNA Interference
  • Receptor, Insulin / genetics
  • Receptor, Insulin / metabolism
  • Signal Transduction
  • Transcription Factors / genetics*
  • Transcription Factors / physiology*
  • Up-Regulation

Substances

  • Caenorhabditis elegans Proteins
  • Forkhead Transcription Factors
  • Insulin
  • Transcription Factors
  • daf-16 protein, C elegans
  • DAF-2 protein, C elegans
  • Receptor, Insulin