The work was outlined to elucidate contribution of soluble factors produced by peripheral neutrophils from healthy pregnant women and those with EPH-gestosis to regulation of expression of surface activation markers by donor lymphocytes. It was found out that in healthy pregnant women neutrophil factors stimulated expression of HLA-DR and CD25 antigens by T lymphocytes. In women with EPH-gestosis neutrophil factors, which comprised soluble antigen of leukocytes 2 (SAL-2), enhanced expression of CD25 antigen in B subset. Presumably, the effects were caused by activation of SAL-2 neutrophil secretion during EPH-gestosis. Impaired neutrophil secretion in women with EPH-gestosis may lead to activation of B lymphocytes through Th2 recruitment and triggering of the autoimmune reactions.