The energy spectrum of fission neutrons in the biological irradiation field of the Kinki University reactor, UTR-KINKI, has been determined by a multi-foil activation analysis coupled with artificial neural network techniques and a Au-foil activation method. The mean neutron energy was estimated to be 1.26 +/- 0.05 MeV from the experimentally determined spectrum. Based on this energy value and other information, the neutron dose rate was estimated to be 19.7 +/- 1.4 cGy/hr. Since this dose rate agrees with that measured by a pair of ionizing chambers (21.4 cGy/hr), we conclude that the mean neutron energy could be estimated with reasonable accuracy in the irradiation field of UTR-KINKI.