Abstract
Benzoxazinoids are secondary metabolites of grasses that function as natural pesticides. While many steps of DIMBOA biosynthesis have been elucidated, the mechanism of the introduction of OCH(3)-group at the C-7 position was unknown. Inhibitor experiments in Triticum aestivum and Zea mays suggest that a 2-oxoglutarate-dependent dioxygenase catalyses the hydroxylation reaction at C-7. Cloning and reverse genetics analysis have identified the Bx6 gene that encodes this enzyme. Bx6 is located in the Bx-gene cluster of maize.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Benzoxazines
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Blotting, Northern
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Chromosome Mapping
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Cloning, Molecular
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DNA Transposable Elements / genetics
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Enzyme Inhibitors / pharmacology
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Evolution, Molecular
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Hydroxylation
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Ketoglutaric Acids / metabolism*
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Methylation
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Molecular Sequence Data
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Mutation
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Oxazines / metabolism*
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Oxygenases / antagonists & inhibitors
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Oxygenases / genetics
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Oxygenases / metabolism*
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Phenotype
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Triticum / enzymology
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Zea mays / enzymology
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Zea mays / genetics
Substances
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Benzoxazines
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DNA Transposable Elements
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Enzyme Inhibitors
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Ketoglutaric Acids
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Oxazines
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Oxygenases
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2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one