A 2-oxoglutarate-dependent dioxygenase is integrated in DIMBOA-biosynthesis

Phytochemistry. 2003 Feb;62(3):371-6. doi: 10.1016/s0031-9422(02)00556-3.

Abstract

Benzoxazinoids are secondary metabolites of grasses that function as natural pesticides. While many steps of DIMBOA biosynthesis have been elucidated, the mechanism of the introduction of OCH(3)-group at the C-7 position was unknown. Inhibitor experiments in Triticum aestivum and Zea mays suggest that a 2-oxoglutarate-dependent dioxygenase catalyses the hydroxylation reaction at C-7. Cloning and reverse genetics analysis have identified the Bx6 gene that encodes this enzyme. Bx6 is located in the Bx-gene cluster of maize.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Benzoxazines
  • Blotting, Northern
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Transposable Elements / genetics
  • Enzyme Inhibitors / pharmacology
  • Evolution, Molecular
  • Hydroxylation
  • Ketoglutaric Acids / metabolism*
  • Methylation
  • Molecular Sequence Data
  • Mutation
  • Oxazines / metabolism*
  • Oxygenases / antagonists & inhibitors
  • Oxygenases / genetics
  • Oxygenases / metabolism*
  • Phenotype
  • Triticum / enzymology
  • Zea mays / enzymology
  • Zea mays / genetics

Substances

  • Benzoxazines
  • DNA Transposable Elements
  • Enzyme Inhibitors
  • Ketoglutaric Acids
  • Oxazines
  • Oxygenases
  • 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one