Structural analysis of conjugated linoleic acid produced by Lactobacillus plantarum, and factors affecting isomer production

Biosci Biotechnol Biochem. 2003 Jan;67(1):179-82. doi: 10.1271/bbb.67.179.

Abstract

An isomer of the conjugated linoleic acid (CLA) produced from linoleic acid by Lactobacillus plantarum was identified as cis-9,trans-11-octadecadienoic acid by proton nuclear magnetic resonance spectroscopy. Together with earlier results, we concluded that the bacterium produces two CLA isomers, cis-9,trans-11- and trans-9,trans-11-octadecadienoic acid from linoleic acid. The addition of L-serine, glucose, AgNO3, or NaCl to the reaction mixture reduced production of the latter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media
  • Enzyme Inhibitors / pharmacology
  • Fatty Acids / chemistry
  • Glucose / pharmacology
  • Isomerism
  • Lactobacillus / chemistry*
  • Lactobacillus / drug effects
  • Lactobacillus / metabolism*
  • Linoleic Acid / biosynthesis
  • Linoleic Acid / chemistry*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Serine / pharmacology
  • Silver Nitrate / pharmacology
  • Sodium Chloride / pharmacology

Substances

  • Culture Media
  • Enzyme Inhibitors
  • Fatty Acids
  • Sodium Chloride
  • Serine
  • Silver Nitrate
  • Linoleic Acid
  • Glucose