Crystallization and preliminary crystallographic studies of juvenile hormone-binding protein from Galleria mellonella haemolymph

Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):519-21. doi: 10.1107/s0907444902022904. Epub 2003 Feb 21.

Abstract

Juvenile hormone-binding protein (JHBP) isolated from Galleria mellonella haemolymph has been crystallized using the hanging-drop method in two polymorphic forms. The best diffracting crystals (2.7 A) are trigonal, space group P3(1)21 (or P3(2)21), with unit-cell parameters a = 110.4, c = 93.9 A. X-ray diffraction data have been collected for the native crystals using synchrotron radiation and cryogenic conditions (100 K).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / chemistry*
  • Carrier Proteins / isolation & purification
  • Crystallization
  • Crystallography, X-Ray
  • Hemolymph / chemistry*
  • Indicators and Reagents
  • Insect Proteins*
  • Lepidoptera / chemistry*

Substances

  • Carrier Proteins
  • Indicators and Reagents
  • Insect Proteins
  • juvenile hormone-binding protein, insect