Objective: To improve Paragonimus westermani chromosome preparation technique.
Methods: After being exposed to colchicine, the gonadal cells of P. westermani were treated by the following procedures: hypotonicity, fixation, dropping onto a slide and staining.
Results: The chromosome number of P. westermani is 22, and the karyotype is 2m + 6Sm + 14t chromosome.
Conclusion: The improved technique of chromosome preparation is feasible to operate and the chromosome is clear enough for karyotype analysis.