Abstract
Objective:
To acquire the variable region gene of light chain of monoclonal antibody against human retinoblastoma.
Methods:
Total RNA were extracted from hybridoma cells secreting specific monoclonal antibody(McAb) against human retinoblastoma(RB), then transcripted reversely into cDNA with random primers. The variable region of the light chain(VL) gene fragments were ampliflied using polymerase chain reaction(PCR) method. Agrose gel electrophoresis was confirmed.
Results:
1.5% agrose gel electrophoresis indicated that VL gene was about 340 base pairs.
Conclusion:
The light chain variable region gene of the McAb against human RB was amplified successfully, which lays a good basis for construction of a recombinant antibody.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / biosynthesis
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Antibodies, Monoclonal / genetics
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Antibodies, Neoplasm / genetics*
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Gene Amplification
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Genes, Immunoglobulin / genetics
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Humans
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Hybridomas / metabolism
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Immunoglobulin Light Chains / biosynthesis
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Immunoglobulin Light Chains / genetics*
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Immunoglobulin Variable Region / biosynthesis
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Immunoglobulin Variable Region / genetics*
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Immunoglobulin kappa-Chains / genetics
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Polymerase Chain Reaction
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Retinal Neoplasms / genetics
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Retinal Neoplasms / immunology
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Retinoblastoma* / genetics
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Retinoblastoma* / immunology
Substances
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Antibodies, Monoclonal
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Antibodies, Neoplasm
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Immunoglobulin Light Chains
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Immunoglobulin Variable Region
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Immunoglobulin kappa-Chains