Abstract
Outer coat protein gene(S8) of RDV was cloned into the transfer vector pVL 1393 to construct a recombinant vector pVL1393-S8. The recombinant vector pVL1393-S8 and the linear baculovirus RP23. LacZ were cotransfected into sf9 cells to produce the recombinant virus RP23-S8. RP23-S8 infected sf9 cells were collected and analysed by SDS-PAGE and Western-blot. The results showed that the S8 gene of RDV was expressed in sf9 cells and the expression level of sf9 cells was higher between 72-96 h after infected.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Baculoviridae / genetics
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Capsid Proteins / biosynthesis*
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Capsid Proteins / genetics
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Cells, Cultured
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Gene Expression
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Genes, Viral*
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Genetic Vectors
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Oryza / virology
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Plant Diseases / virology
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Plant Viruses / genetics
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Recombinant Proteins / genetics
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Reoviridae / genetics*
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Spodoptera / cytology
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Spodoptera / metabolism*
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Transformation, Bacterial
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Viral Proteins / biosynthesis*
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Viral Proteins / genetics
Substances
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Capsid Proteins
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Recombinant Proteins
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Viral Proteins
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S8 protein, rice gall dwarf virus