[Expression of rice dwarf virus outer coat protein gene(S8) in insect cells]

Wei Sheng Wu Xue Bao. 2001 Apr;41(2):162-6.
[Article in Chinese]

Abstract

Outer coat protein gene(S8) of RDV was cloned into the transfer vector pVL 1393 to construct a recombinant vector pVL1393-S8. The recombinant vector pVL1393-S8 and the linear baculovirus RP23. LacZ were cotransfected into sf9 cells to produce the recombinant virus RP23-S8. RP23-S8 infected sf9 cells were collected and analysed by SDS-PAGE and Western-blot. The results showed that the S8 gene of RDV was expressed in sf9 cells and the expression level of sf9 cells was higher between 72-96 h after infected.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baculoviridae / genetics
  • Capsid Proteins / biosynthesis*
  • Capsid Proteins / genetics
  • Cells, Cultured
  • Gene Expression
  • Genes, Viral*
  • Genetic Vectors
  • Oryza / virology
  • Plant Diseases / virology
  • Plant Viruses / genetics
  • Recombinant Proteins / genetics
  • Reoviridae / genetics*
  • Spodoptera / cytology
  • Spodoptera / metabolism*
  • Transformation, Bacterial
  • Viral Proteins / biosynthesis*
  • Viral Proteins / genetics

Substances

  • Capsid Proteins
  • Recombinant Proteins
  • Viral Proteins
  • S8 protein, rice gall dwarf virus