[Recombinant Salmonella typhimurium anticaries vaccine. II. Construction and confirmation of expressing plasmid carrying a region of S. mutans pac gene]

Hua Xi Kou Qiang Yi Xue Za Zhi. 2001 Jun;19(3):139-41.
[Article in Chinese]

Abstract

Objective: The objective of this study is to construct prokaryotic expressing plasmids carrying A region of S. mutans pac gene.

Methods: The A region of pac gene was amplified by using PCR from plasmid pPC41 containing S. mutans surface protein pac gene. The new expression plasmid was constructed by using A region of pac gene and expressing plasmids pET17-b and directed cloning technique. The recombinant plasmid was identified by agarose gel electrophoresis after it was cut by using BamHI and EcoRV.

Results: The expressing plasmid carrying pac gene A region was acquired.

Conclusion: The recombinant plasmid provides useful experimental materials for further research work.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dental Caries / prevention & control*
  • Gene Expression
  • Genes, Bacterial*
  • Plasmids / genetics*
  • Recombination, Genetic
  • Salmonella Vaccines / genetics*
  • Salmonella Vaccines / immunology
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / immunology
  • Streptococcus mutans / genetics*

Substances

  • Salmonella Vaccines