Objective: To sequence the Streptococcus mutans surface protein pac gene's upstream.
Methods: The PCR autosequence kit and autosequence instrument ABI310 were used for sequence.
Results: The 517 base pairs sequence of the pac gene's upstream is sequenced.
Conclusion: The sequence of pac gene's upstream will provide useful information to construct the recombinant Salmonella typhimurium anticaries vaccine.