Abstract
The orphan nuclear receptor steroidogenic factor-1 (SF-1) plays pivotal roles in the development and function of steroidogenic organs. Here we describe the differential effect of protein kinase A (PKA) on coregulation of SF-1 dependent transcription by two p160 family members, p300/CBP co-integrator-associated protein (p/CIP) and transcription intermediary factor-2 (TIF2). Thus, whereas p/CIP-stimulated SF-1 dependent transcription is further potentiated by PKA, we show that activation of PKA leads to selective downregulation of TIF2 protein and a subsequent repression of TIF2 coactivator function. Using a yeast two-hybrid screen we also identified a novel zinc finger containing protein, which interacts with SF-1 via the AF-2 domain.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Animals
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Cyclic AMP-Dependent Protein Kinases / metabolism*
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DNA-Binding Proteins / physiology*
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Fushi Tarazu Transcription Factors
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Histone Acetyltransferases
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Homeodomain Proteins
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Humans
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Nuclear Receptor Coactivator 2
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Nuclear Receptor Coactivator 3
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Protein Structure, Tertiary / physiology
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Receptors, Cytoplasmic and Nuclear
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Steroidogenic Factor 1
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Trans-Activators / metabolism*
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Transcription Factors / metabolism*
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Transcription Factors / physiology*
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Transcription, Genetic / physiology*
Substances
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DNA-Binding Proteins
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Fushi Tarazu Transcription Factors
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Homeodomain Proteins
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NCOA2 protein, human
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NR5A1 protein, human
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Nuclear Receptor Coactivator 2
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Receptors, Cytoplasmic and Nuclear
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Steroidogenic Factor 1
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Trans-Activators
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Transcription Factors
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Histone Acetyltransferases
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NCOA3 protein, human
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Nuclear Receptor Coactivator 3
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Cyclic AMP-Dependent Protein Kinases