Differential regulation of SF-1-cofactor interactions

Endocr Res. 2002 Nov;28(4):505-13. doi: 10.1081/erc-120016830.

Abstract

The orphan nuclear receptor steroidogenic factor-1 (SF-1) plays pivotal roles in the development and function of steroidogenic organs. Here we describe the differential effect of protein kinase A (PKA) on coregulation of SF-1 dependent transcription by two p160 family members, p300/CBP co-integrator-associated protein (p/CIP) and transcription intermediary factor-2 (TIF2). Thus, whereas p/CIP-stimulated SF-1 dependent transcription is further potentiated by PKA, we show that activation of PKA leads to selective downregulation of TIF2 protein and a subsequent repression of TIF2 coactivator function. Using a yeast two-hybrid screen we also identified a novel zinc finger containing protein, which interacts with SF-1 via the AF-2 domain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • DNA-Binding Proteins / physiology*
  • Fushi Tarazu Transcription Factors
  • Histone Acetyltransferases
  • Homeodomain Proteins
  • Humans
  • Nuclear Receptor Coactivator 2
  • Nuclear Receptor Coactivator 3
  • Protein Structure, Tertiary / physiology
  • Receptors, Cytoplasmic and Nuclear
  • Steroidogenic Factor 1
  • Trans-Activators / metabolism*
  • Transcription Factors / metabolism*
  • Transcription Factors / physiology*
  • Transcription, Genetic / physiology*

Substances

  • DNA-Binding Proteins
  • Fushi Tarazu Transcription Factors
  • Homeodomain Proteins
  • NCOA2 protein, human
  • NR5A1 protein, human
  • Nuclear Receptor Coactivator 2
  • Receptors, Cytoplasmic and Nuclear
  • Steroidogenic Factor 1
  • Trans-Activators
  • Transcription Factors
  • Histone Acetyltransferases
  • NCOA3 protein, human
  • Nuclear Receptor Coactivator 3
  • Cyclic AMP-Dependent Protein Kinases