[MTHFR gene expression in EB virus transformed human B lymphoblasts]

Abstract

In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase(MTHFR) gene in Epstein-Barr virus(EBV) transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT-PCR protocol and analysed by polyacrylamide gel electrophoresis(PAGE). One of the cDNA segments was sequenced. Results showed MTHFR gene was expressed in human B lymphoblast cell line. The cDNA sequence was almost the same as that of human liver cDNA from Genbank.