Prostate biopsy is a fundamental step in the diagnostic flow chart of prostate cancer. We have evaluated the influence of the number of cores taken on the tolerability and morbidity of the transperineal ultrasound-guided prostate biopsy. We have compared a group of 240 pts undergone 16 core prostate biopsy to an historical cohort of 100 pts undergone 10 core prostate biopsy. All the patients in both groups have been investigated about tolerability and morbidity of the procedure. No significant differences emerge between the two groups, and then we conclude that the number of cores taken does not influence the safety and compliance to the transperineal biopsy. This is a significant advantage compared to transrectal biopsy, as the number of cores influences the detection rate of prostate carcinoma.