Carbohydrase activities of the rolC-gene transformed and non-transformed ginseng cultures

Abstract

The levels of activity of beta-D-glucosidase, alpha-D-mannosidase, alpha- and beta-D-galactosidase, 1,3-, 1,6-, 1,4-beta-D-glucanases, 1,4-alpha-D-glucanase, fucoidanhydrolase and agarase were measured in extracts of non-transgenic and transgenic Panax ginseng cultures transformed with the rolC gene. Significantly increased levels of activity of beta- and alpha-D-galactosidases and 1,3-beta-D-glucanase were detected in rolC-gene transformed cells, compared to the control non-transformed cells, while levels of activity of other enzymes were unchanged. These, as well as the gel-permeation experiments, revealed that transformation of ginseng cells by the rolC gene could significantly affect activity of some carbohydrases and production of their molecular forms.