Abstract
The binding properties of beta-AgTX, a snake pre-synaptic toxin, membranes and its effect on transmitter release from cerebrocortical synaptosomes were investigated. Assay of [125I]-beta-AgTX binding to rat synaptic membrane revealed a high affinity binding site for the toxin within the synaptic membrane. Preincubation with beta-AgTX inhibited K+-evoked Ca2+-dependent glutamate release from synaptosomes in a concentration-dependent manner, as determined by an on-line enzyme-linked fluorometric assay. The toxin also blocked the Ca2+-dependent release of other transmitters, aspartate, glycine, and GABA induced by K+-depolarization. However, Ca2+-ionophore, ionomycin-stimulated Ca2+-dependent transmitter release was not significantly affected by beta-AgTX, indicating that the toxin inhibits transmitter release by reducing the entry of Ca2+ into cytoplasm. It is suggested that beta-AgTX-binding site in synaptic membrane is related to the release of a variety of transmitters.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aspartic Acid / metabolism
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Binding Sites / physiology
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Calcium / metabolism
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Calcium Signaling / drug effects*
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Calcium Signaling / physiology
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Cerebral Cortex / drug effects*
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Cerebral Cortex / metabolism
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Crotalid Venoms / pharmacology*
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Glutamic Acid / metabolism
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Glycine / metabolism
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Iodine Radioisotopes
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Male
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Models, Biological
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Neurotoxins / pharmacology*
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Neurotransmitter Agents / metabolism*
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Presynaptic Terminals / drug effects*
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Presynaptic Terminals / metabolism
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Protein Binding / physiology
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Radioligand Assay
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Rats
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Rats, Sprague-Dawley
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Subcellular Fractions
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Synaptic Membranes / drug effects
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Synaptic Membranes / metabolism*
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Synaptic Transmission / drug effects*
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Synaptic Transmission / physiology
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Synaptosomes
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gamma-Aminobutyric Acid / metabolism
Substances
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Crotalid Venoms
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Iodine Radioisotopes
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Neurotoxins
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Neurotransmitter Agents
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Aspartic Acid
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Glutamic Acid
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gamma-Aminobutyric Acid
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beta-agkistrodotoxin
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Calcium
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Glycine