Abstract
Laser dissection microscopy was applied to isolate endothelial cells from tumors obtained from mice treated with TNF-alpha. RNA integrity was demonstrated from whole sections and dissected cells after acetone fixation and hematoxylin staining. RT-PCR for GAPDH, CD31, VCAM-1, ICAM-1, and E-selectin was successfully performed on these samples. These data demonstrate the feasibility of analyzing local endothelial cell responses in diseased tissues at the level of gene expression.
MeSH terms
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Animals
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E-Selectin / genetics
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Endothelium, Vascular / pathology
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Endothelium, Vascular / physiopathology*
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Intercellular Adhesion Molecule-1 / genetics
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Lasers
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Mice
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Microscopy
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Neoplasms, Experimental / blood supply
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Neoplasms, Experimental / genetics
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Platelet Endothelial Cell Adhesion Molecule-1 / genetics
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Reverse Transcriptase Polymerase Chain Reaction*
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Tumor Cells, Cultured
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Tumor Necrosis Factor-alpha / pharmacology
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Vascular Cell Adhesion Molecule-1 / genetics
Substances
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E-Selectin
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Platelet Endothelial Cell Adhesion Molecule-1
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Tumor Necrosis Factor-alpha
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Vascular Cell Adhesion Molecule-1
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Intercellular Adhesion Molecule-1