In glial cell lines from the central (CG4, C6) and peripheral (MSC80, CR3a1, and CR1b4) nervous systems, glucocorticoid receptor (GR) messenger RNA was clearly detected and was consistent with significant GR binding and transcriptional activity. Mineralocorticosteroid receptor mRNA was less abundant, with no corresponding binding and lack of transcriptional activity. Other steroid receptors were not significantly detected.