Caged RNase: photoactivation of the enzyme from perfect off-state by site-specific incorporation of 2-nitrobenzyl moiety

Takashi Hiraoka; Itaru Hamachi

doi:10.1016/s0960-894x(02)00825-9

Caged RNase: photoactivation of the enzyme from perfect off-state by site-specific incorporation of 2-nitrobenzyl moiety

Bioorg Med Chem Lett. 2003 Jan 6;13(1):13-5. doi: 10.1016/s0960-894x(02)00825-9.

Authors

Takashi Hiraoka¹, Itaru Hamachi

Affiliation

¹ Institute for Fundamental Research of Organic Chemistry (IFOC), Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.

PMID: 12467607
DOI: 10.1016/s0960-894x(02)00825-9

Abstract

Photo-triggered activation of semisynthetic Ribonuclease S' from a perfect off-state was successfully carried out by incorporation of photo-labile caged moiety into a proximity to the active site.

MeSH terms

Amino Acid Sequence
Binding Sites
Mutagenesis, Site-Directed
Mutation
Nitrobenzenes / chemistry*
Peptide Fragments / chemistry
Peptide Fragments / radiation effects
Photolysis
Ribonucleases / chemistry*
Ribonucleases / metabolism
Ribonucleases / radiation effects

Substances

Nitrobenzenes
Peptide Fragments
ribonuclease S-peptide
nitrobenzene
Ribonucleases
ribonuclease S