Aims: Optimization of Penicillin G acylase (PAC) production from a novel isolate of Bacillus sp.
Methods: Fermentation medium for PAC production was optimized using a two-level fractional factorial design with seven components.
Results: A maximum production of 9.5 U ml(-1) of PAC was obtained in an optimized medium containing (g l(-1): K2HPO4, 1.0; MgSO4.7H2O, 0.1; CaCl2.2H2O, 0.1; PAA, 2.0; tryptone, 5.0; yeast extract, 3.0; and sucrose, 50.0.
Significance and impact of the study: The two-step medium optimization resulted in a twofold increase in PAC production. Since the strain Bacillus sp. PGS10 produces a high level of PAC, it could be a potential candidate for industrial production of PAC.