Photoinduced cleavage by a rhodium complex at G.U mismatches and exposed guanines in large and small RNAs

Biochimie. 2002 Sep;84(9):859-68. doi: 10.1016/s0300-9084(02)01403-7.

Abstract

Photoinduced cleavage reactions by the rhodium complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)(3)(3+)] with three RNA hairpins, r(GGGGU UCGCUC CACCA) (16 nucleotide, tetraloop(Ala2)), r(GGGGCUAUAGCUCUAGCUC CACCA) (24 nucleotide, microhelix(Ala)), and r(GGCGGUUAGAUAUCGCC) (17 nucleotide, 790 loop), and full-length (1542 nucleotide) 16S rRNA from Escherichia coli were investigated. The cleavage reactions were monitored by gel electrophoresis and the sites of cleavage by Rh(DIP)(3)(3+) were determined by comparisons with chemical or enzymatic sequencing reactions. In general, RNA backbone scission by the metal complex was induced at G.U mismatches and at exposed G residues. The cleavage activity was observed on the three small RNA hairpins as well as on the isolated 1542-nucleotide ribosomal RNA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Composition
  • Base Pair Mismatch*
  • Base Sequence
  • Binding Sites
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Guanine Nucleotides / chemistry*
  • Nucleic Acid Conformation
  • Organometallic Compounds / chemistry*
  • Phosphorus Isotopes
  • Photochemistry
  • RNA / chemistry*
  • RNA, Bacterial / chemistry
  • RNA, Ribosomal, 16S / chemistry
  • RNA, Transfer / chemistry
  • Rhodium / chemistry*
  • Sensitivity and Specificity
  • Thermodynamics
  • Uracil Nucleotides / chemistry*

Substances

  • Guanine Nucleotides
  • Organometallic Compounds
  • Phosphorus Isotopes
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Uracil Nucleotides
  • RNA
  • RNA, Transfer
  • Rhodium