Retention of bioactive ligand conformation in a gaseous protein-trisaccharide complex

Elena N Kitova; Weijie Wang; David R Bundle; John S Klassen

doi:10.1021/ja0281380

Retention of bioactive ligand conformation in a gaseous protein-trisaccharide complex

J Am Chem Soc. 2002 Nov 27;124(47):13980-1. doi: 10.1021/ja0281380.

Authors

Elena N Kitova¹, Weijie Wang, David R Bundle, John S Klassen

Affiliation

¹ Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.

PMID: 12440881
DOI: 10.1021/ja0281380

Abstract

Arrhenius parameters, obtained with the blackbody infrared radiative dissociation technique, are reported for the dissociation of gaseous protonated complexes of a single-chain variable fragment (scFv) of the monoclonal antibody Se155-4 with structurally constrained trisaccharide ligands that resemble the bioactive conformer. The similarity in the dissociation activation energies measured for the +10 charge-state complexes of the constrained ligands and the native trisaccharide is evidence that the bioactive conformation of the native ligand is retained in the gas phase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Gases
Immunoglobulin Fragments / chemistry*
Kinetics
Molecular Sequence Data
Protein Conformation
Spectrometry, Mass, Electrospray Ionization
Static Electricity
Trisaccharides / chemistry*

Substances

Antibodies, Monoclonal
Gases
Immunoglobulin Fragments
Trisaccharides