Leptin, secreted by white adipocytes, has profound feeding, metabolic, and neuroendocrine effects. Leptin acts on the brain, but specific anatomical sites and pathways responsible for mediating these effects are still unclear. We have systematically examined the distribution of leptin receptor containing neurons in the porcine hypothalamus by means of immunohistochemical staining methods. Leptin receptor immunoreactivity (OBR-IR) was observed in both the preoptic area and anterior hypothalamic area. No immunoreactive structures were found in the median eminence. Only single, small neurons were observed in the arcuate nucleus. The most abundant OBR-IR cell bodies were located in the supraoptic nucleus. In the paraventricular nucleus, OBR-IR neurons were moderate in number. Single, dispersed neurons were found in the ventromedial nucleus. These findings indicate that there are distinct OBR-IR neuronal populations in the porcine hypothalamus and leptin not only plays an integrative role in feeding behavior, but also in neuroendocrine activity.