Abstract
In Arabidopsis, root hairs are formed only from a set of epidermal cells named trichoblasts or hair-forming cells. Previous studies showed CAPRICE (CPC) promotes differentiation of hair-forming cells by controlling a negative regulator, GLABRA2 (GL2), which is preferentially expressed in hairless cells. Here, we show that CPC is also predominantly expressed in the hairless cells, but not in the neighboring hair-forming cells, and that CPC protein moves to the hair-forming cells and represses the GL2 expression. We also show that the N terminus of bHLH protein interacts with CPC and is responsible for the GL2 expression. We propose a model in which CPC plays a key role in the fate-determination of hair-forming cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / cytology
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Arabidopsis / genetics
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Arabidopsis / physiology*
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Arabidopsis Proteins / metabolism
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Cell Differentiation / physiology*
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Gene Expression Regulation, Plant
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Genes, Plant
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Genes, Reporter
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Homeodomain Proteins / metabolism
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In Situ Hybridization
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Phenotype
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Plant Epidermis / cytology
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Plant Epidermis / physiology*
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Plant Proteins / metabolism*
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Plant Roots / cytology
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Plant Roots / growth & development*
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Plants, Genetically Modified
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Promoter Regions, Genetic
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Protein Binding
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Proto-Oncogene Proteins c-myb / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Two-Hybrid System Techniques
Substances
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ATR1 protein, Arabidopsis
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Arabidopsis Proteins
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CPC protein, Arabidopsis
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GL2 protein, Arabidopsis
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Homeodomain Proteins
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Plant Proteins
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Proto-Oncogene Proteins c-myb
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Recombinant Fusion Proteins