Whole inactivated SIV virion vaccines with functional envelope glycoproteins: safety, immunogenicity, and activity against intrarectal challenge

J Med Primatol. 2002 Aug;31(4-5):205-16. doi: 10.1034/j.1600-0684.2002.02007.x.

Abstract

A novel type of whole inactivated simian immunodeficiency virus (SIV) virion vaccine immunogen with functional envelope glycoproteins was evaluated, without adjuvant, in rhesus macaques. Immunogens included purified inactivated virions of SIVmac239, a designed mutant of SIVmac239 with gp120 carbohydrate attachment sites deleted (SIVmac239 g4,5), and SIVmneE11S. The vaccines were noninfectious, safe, and immunogenic, inducing antibody responses and cellular responses, including responses by CD8+ lymphocytes. Interpretation of protective efficacy following intrarectal challenge was complicated by incomplete take of the challenge in some SIV naïve controls.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Administration, Rectal
  • Animals
  • Antibodies, Viral / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Chromatography, High Pressure Liquid
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gene Products, env / immunology*
  • Lymphocyte Activation
  • Macaca mulatta / immunology*
  • Macaca mulatta / virology*
  • Male
  • RNA, Viral / blood
  • SAIDS Vaccines / adverse effects*
  • SAIDS Vaccines / immunology*
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Simian Acquired Immunodeficiency Syndrome / prevention & control
  • Simian Acquired Immunodeficiency Syndrome / virology
  • Simian Immunodeficiency Virus / immunology*
  • Simian Immunodeficiency Virus / physiology
  • Time Factors
  • Vaccines, Attenuated / adverse effects*
  • Vaccines, Attenuated / immunology*
  • Viral Load

Substances

  • Antibodies, Viral
  • Gene Products, env
  • RNA, Viral
  • SAIDS Vaccines
  • Vaccines, Attenuated