A spectrophotometric assay for feruloyl esterases

Anal Biochem. 2002 Oct 1;309(1):96-101. doi: 10.1016/s0003-2697(02)00241-5.

Abstract

We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method proposed here is accurate, rapid, and easy to perform.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carboxylic Ester Hydrolases / chemistry
  • Carboxylic Ester Hydrolases / metabolism*
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Thin Layer
  • Endopeptidases / chemistry
  • Endopeptidases / metabolism
  • Esterases / chemistry
  • Esterases / classification
  • Esterases / metabolism
  • Hydrolysis
  • Kinetics
  • Lipase / chemistry
  • Lipase / metabolism
  • Nitrophenols / analysis
  • Nitrophenols / metabolism
  • Octoxynol / chemistry
  • Spectrophotometry, Ultraviolet / methods*
  • Substrate Specificity

Substances

  • Nitrophenols
  • Octoxynol
  • Esterases
  • Carboxylic Ester Hydrolases
  • Lipase
  • feruloyl esterase
  • Endopeptidases
  • 4-nitrophenol