Abstract
We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method proposed here is accurate, rapid, and easy to perform.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Carboxylic Ester Hydrolases / chemistry
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Carboxylic Ester Hydrolases / metabolism*
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Chromatography, High Pressure Liquid / methods
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Chromatography, Thin Layer
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Endopeptidases / chemistry
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Endopeptidases / metabolism
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Esterases / chemistry
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Esterases / classification
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Esterases / metabolism
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Hydrolysis
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Kinetics
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Lipase / chemistry
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Lipase / metabolism
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Nitrophenols / analysis
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Nitrophenols / metabolism
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Octoxynol / chemistry
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Spectrophotometry, Ultraviolet / methods*
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Substrate Specificity
Substances
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Nitrophenols
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Octoxynol
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Esterases
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Carboxylic Ester Hydrolases
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Lipase
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feruloyl esterase
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Endopeptidases
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4-nitrophenol