DNA microarray analysis of differentially expressed genes responsive to bisphenol A, an alkylphenol derivative, in an in vitro mouse Sertoli cell model

Jpn J Pharmacol. 2002 Aug;89(4):413-6. doi: 10.1254/jjp.89.413.

Abstract

To identify genes elicited by bisphenol A (BPA) in Sertoli cells, we carried out a microarray analysis of TTE3 cells (a mouse Sertoli cell line) treated with BPA. BPA (100, 200 and 400 microM) induced cell death concentration-dependently, with levels being 25%, 33% and 96%, respectively. Of the 1,081 genes analyzed, 3 genes showed decreased levels of expression while the remaining 10 genes showed increased levels in the cells treated with a subtoxic dose of BPA (200 microM). The expressions of six genes were confirmed by the TaqMan assay. These findings suggest that DNA microarray analysis is a useful tool for investigating the molecular mechanisms of the toxic effects of BPA in testicular cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzhydryl Compounds
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Gene Expression Regulation / drug effects*
  • Gene Expression Regulation / physiology
  • Male
  • Mice
  • Oligonucleotide Array Sequence Analysis / methods*
  • Phenol / pharmacology
  • Phenols / pharmacology*
  • Sertoli Cell Tumor / metabolism*
  • Testicular Neoplasms / metabolism*

Substances

  • Benzhydryl Compounds
  • Phenols
  • Phenol
  • bisphenol A