Abstract
We succeeded in cloning the gene, termed d-ddb1, for a Drosophila homolog of the p127 subunit of the human damage-specific DNA-binding protein, thought to recognize (6-4) photoproducts and related structures. In Drosophila, the gene product (D-DDB1) also appeared to play a role as a repair factor, d-ddb1 knockout Kc cells generated with a RNAi method being sensitive to UV. In addition, UV or methyl methanesulfonate treatment increased d-ddb1 transcripts. However, we found that the gene is controlled by the DRE/DREF system, which is generally responsible for activating the promoters of proliferation-related genes. Moreover, during Drosophila development, the transcription of d-ddb1 changed greatly, with the highest levels in unfertilized eggs, indicating that external injury to DNA is not essential to D-DDB1 function. Interestingly, as with UV irradiation-induced transfer of D-DDB1 to the nucleus from the cytoplasm, during spermatogenesis the protein transiently shifted from one cell compartment to the other. The results indicate that D-DDB1 not only contributes to the DNA repair system, but also has a role in cell proliferation and development.
MeSH terms
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5' Flanking Region / genetics
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Animals
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Base Sequence
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Binding Sites / genetics
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Cell Line
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DNA Replication / genetics
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Drosophila Proteins*
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Drosophila melanogaster / cytology
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Drosophila melanogaster / genetics*
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Embryo, Nonmammalian / metabolism
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Embryonic Development
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Female
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / radiation effects
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Gene Expression Regulation, Developmental
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Gene Silencing
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Luciferases / genetics
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Luciferases / metabolism
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Male
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Methyl Methanesulfonate / pharmacology
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Molecular Sequence Data
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Mutation
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RNA, Bacterial / genetics
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RNA, Bacterial / physiology
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RNA, Double-Stranded / genetics
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RNA, Double-Stranded / physiology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Response Elements / genetics
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Response Elements / physiology*
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Sequence Deletion
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Time Factors
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Ultraviolet Rays
Substances
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DNA-Binding Proteins
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Dref protein, Drosophila
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Drosophila Proteins
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RNA I
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RNA, Bacterial
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RNA, Double-Stranded
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RNA, Messenger
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Recombinant Fusion Proteins
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Transcription Factors
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Methyl Methanesulfonate
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Luciferases