Objective: To study effective method of showing the microvascular architecture of human and rabbit skin flaps.
Methods: According to endogenous alkaline phosphatase activity, Gomori-Takamatsu method and NBT/BCIP method were used to observe the microvascular architecture.
Results: Both methods had capability to demonstrate the microvascular architecture of the human skin flap, whereas the examining results of rabbits were negative.
Conclusions: Great difference of endogeneous alkaline phosphatase activity exists in different animals. Gomori-Takamatsu method and NBT/BCIP method are useful in study of microvascular architecture of human skin flaps.