Production of recombinant human calcitonin from silkworm (B. mori) larvae infected by baculovirus

Protein Pept Lett. 2002 Aug;9(4):323-9. doi: 10.2174/0929866023408689.

Abstract

A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.

MeSH terms

  • Animals
  • Baculoviridae / genetics
  • Baculoviridae / physiology*
  • Bombyx / genetics
  • Bombyx / physiology*
  • Bombyx / virology
  • Calcitonin / genetics
  • Calcitonin / metabolism*
  • Calcitonin / pharmacology
  • Genes, Reporter
  • Hemolymph / chemistry
  • Humans
  • Hypercalcemia / chemically induced
  • Larva / physiology
  • Rats
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Fusion Proteins / pharmacology

Substances

  • Recombinant Fusion Proteins
  • Calcitonin