Abstract
Mitochondrial transcription factor A (mtTFA) is necessary for both transcription and maintenance of mtDNA, and is also one of the high mobility group (HMG) proteins that preferentially binds to cisplatin-damaged DNA. In this study we confirmed the preferential binding of mtTFA to cisplatin-damaged DNA, and also discovered that mtTFA binds to oxidatively damaged DNA. The affinity for oxidatively damaged DNA of mtTFA is higher for A/8-oxo-dG and C/8-oxo-dG than for G/8-oxo-dG and T/8-oxo-dG. Our findings suggest that mtTFA plays an important role in the recognition of oxidative DNA damage.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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8-Hydroxy-2'-Deoxyguanosine
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Cisplatin / pharmacology
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Cross-Linking Reagents / pharmacology
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DNA / metabolism*
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DNA Damage
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DNA, Complementary / metabolism
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DNA-Binding Proteins*
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Deoxyguanosine / analogs & derivatives
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Deoxyguanosine / pharmacology
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Glutathione Transferase / metabolism
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Humans
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Mitochondria / metabolism*
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Mitochondrial Proteins*
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Nuclear Proteins*
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Oxygen / metabolism
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Plasmids / metabolism
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Protein Binding
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Reactive Oxygen Species
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Recombinant Fusion Proteins / metabolism
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Trans-Activators*
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Transcription Factors / chemistry
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Transcription Factors / metabolism*
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Xenopus Proteins*
Substances
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Cross-Linking Reagents
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DNA, Complementary
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DNA-Binding Proteins
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Mitochondrial Proteins
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Nuclear Proteins
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Reactive Oxygen Species
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Recombinant Fusion Proteins
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TFAM protein, human
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Trans-Activators
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Transcription Factors
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XL-MTTFA protein, Xenopus
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Xenopus Proteins
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mitochondrial transcription factor A
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8-Hydroxy-2'-Deoxyguanosine
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DNA
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Glutathione Transferase
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Deoxyguanosine
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Cisplatin
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Oxygen