Human mitochondrial transcription factor A binds preferentially to oxidatively damaged DNA

Biochem Biophys Res Commun. 2002 Jul 26;295(4):945-51. doi: 10.1016/s0006-291x(02)00757-x.

Abstract

Mitochondrial transcription factor A (mtTFA) is necessary for both transcription and maintenance of mtDNA, and is also one of the high mobility group (HMG) proteins that preferentially binds to cisplatin-damaged DNA. In this study we confirmed the preferential binding of mtTFA to cisplatin-damaged DNA, and also discovered that mtTFA binds to oxidatively damaged DNA. The affinity for oxidatively damaged DNA of mtTFA is higher for A/8-oxo-dG and C/8-oxo-dG than for G/8-oxo-dG and T/8-oxo-dG. Our findings suggest that mtTFA plays an important role in the recognition of oxidative DNA damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Cisplatin / pharmacology
  • Cross-Linking Reagents / pharmacology
  • DNA / metabolism*
  • DNA Damage
  • DNA, Complementary / metabolism
  • DNA-Binding Proteins*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / pharmacology
  • Glutathione Transferase / metabolism
  • Humans
  • Mitochondria / metabolism*
  • Mitochondrial Proteins*
  • Nuclear Proteins*
  • Oxygen / metabolism
  • Plasmids / metabolism
  • Protein Binding
  • Reactive Oxygen Species
  • Recombinant Fusion Proteins / metabolism
  • Trans-Activators*
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism*
  • Xenopus Proteins*

Substances

  • Cross-Linking Reagents
  • DNA, Complementary
  • DNA-Binding Proteins
  • Mitochondrial Proteins
  • Nuclear Proteins
  • Reactive Oxygen Species
  • Recombinant Fusion Proteins
  • TFAM protein, human
  • Trans-Activators
  • Transcription Factors
  • XL-MTTFA protein, Xenopus
  • Xenopus Proteins
  • mitochondrial transcription factor A
  • 8-Hydroxy-2'-Deoxyguanosine
  • DNA
  • Glutathione Transferase
  • Deoxyguanosine
  • Cisplatin
  • Oxygen