Role of the high-affinity zinc uptake znuABC system in Salmonella enterica serovar typhimurium virulence

Infect Immun. 2002 Aug;70(8):4721-5. doi: 10.1128/IAI.70.8.4721-4725.2002.

Abstract

The Salmonella enterica serovar Typhimurium znuABC genes encoding a high-affinity zinc uptake system and its regulatory zur gene have been cloned. Salmonella serovar Typhimurium zur and znuC knockout mutants have been constructed by marker exchange. The 50% lethal dose of the znuC mutant increased when either orally or intraperitoneally inoculated in BALB/c mice, while virulence of the zur mutant decreased only when mice were intraperitoneally challenged.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cation Transport Proteins*
  • Cell Line
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Disease Models, Animal
  • Epithelial Cells / microbiology
  • Escherichia coli Proteins*
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Mutagenesis
  • Phenotype
  • Salmonella Infections / microbiology*
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / pathogenicity*
  • Swine
  • Virulence
  • Zinc / metabolism*

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • Cation Transport Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • ZnuC protein, Salmonella enterica
  • Zur protein, E coli
  • Zinc