Telomere Shortening of MCF-7 Cells Caused by Antisense Telomerase cDNA

Xiao-Wei Zhang; Hai-Ni Liao; Tan-Jun Tong

Telomere Shortening of MCF-7 Cells Caused by Antisense Telomerase cDNA

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(5):527-530.

Authors

Xiao-Wei Zhang¹, Hai-Ni Liao, Tan-Jun Tong

Affiliation

¹ Department of Biochemistry and Molecular Biology, Beijing Medical University, Beijing 100083, China. biochem@mail.bjmu.edu.cn

PMID: 12114965

Abstract

pAdE(1)CMVITREXneo is a novel adenovirus vector which can integrate foreign genes into genome of host cells. The cDNA of human telomerase RNA was integrated inversely into the vector via antisense oligonucleotide technique. Antisense recombinant virus (vAdT-AAV) was obtained by cotransfecting pAdT and pBHG(11) into 293 cells. The telomere length of breast cancer MCF-7 cells was found shortened after the transfection by vAdT-AAV.