Activation of the Sarcophaga lectin gene promoter in transgenic Drosophila

Arch Insect Biochem Physiol. 2002 Jul;50(3):131-8. doi: 10.1002/arch.10039.

Abstract

We established transgenic Drosophila strains in which the lacZ gene was expressed under the control of the 5'-upstream regulatory region of the Sarcophaga lectin gene promoter (3.1 kbp). The reporter gene was expressed in the fat bodies of the transgenic larvae when they were immunized by body pricking or treatment with Escherichia coli, which was the same as the Sarcophaga lectin gene expression in Sarcophaga larvae. However, the same reporter gene was found to be expressed constitutively in the digestive tracts of the transgenic larvae even without immunization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Anti-Bacterial Agents / immunology
  • Cell Line
  • Depsipeptides*
  • Diptera / genetics
  • Diptera / metabolism*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / immunology
  • Escherichia coli / immunology
  • Genes, Reporter
  • Insect Proteins*
  • Lectins / genetics*
  • Lectins, C-Type*
  • Peptides*
  • Promoter Regions, Genetic*
  • Staphylococcus aureus / immunology
  • Tissue Distribution
  • Transcriptional Activation*
  • Transgenes
  • beta-Galactosidase / genetics

Substances

  • 20kDa lectin protein, Sarcophaga
  • Anti-Bacterial Agents
  • Depsipeptides
  • Insect Proteins
  • Lectins
  • Lectins, C-Type
  • Peptides
  • beauvericin
  • beta-Galactosidase