Analysis of transactivation region of Elf-1 by using a yeast one-hybrid system

Chiharu Nishiyama; Kyoko Takahashi; Yasuyuki Ohtake; Toyokazu Yokota; Ko Okumura; Hideoki Ogawa; Chisei Ra

doi:10.1271/bbb.66.1105

Analysis of transactivation region of Elf-1 by using a yeast one-hybrid system

Biosci Biotechnol Biochem. 2002 May;66(5):1105-7. doi: 10.1271/bbb.66.1105.

Authors

Chiharu Nishiyama¹, Kyoko Takahashi, Yasuyuki Ohtake, Toyokazu Yokota, Ko Okumura, Hideoki Ogawa, Chisei Ra

Affiliation

¹ Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan. chinishi@med.juntendo.ac.jp

PMID: 12092822
DOI: 10.1271/bbb.66.1105

Abstract

The transcriptional regulatory region of Elf-1 was analyzed by the combination of a yeast one-hybrid system and site-directed mutagenesis. This analysis enabled us to map an activation region between 85-175 of Elf-1.

MeSH terms

Ephrin-A2 / genetics*
Mutagenesis, Site-Directed
Saccharomyces cerevisiae / genetics
Transcriptional Activation*
Two-Hybrid System Techniques

Substances

Ephrin-A2