Calcineurin plays different roles in group II metabotropic glutamate receptor- and NMDA receptor-dependent long-term depression

J Neurosci. 2002 Jun 15;22(12):5034-41. doi: 10.1523/JNEUROSCI.22-12-05034.2002.

Abstract

We investigated metabotropic glutamate receptor (mGluR)-dependent long-term depression (LTD) in hippocampal CA1 pyramidal neurons of 6- to 8-d-old [postnatal days 6-8 (P6-P8)] and 21- to 25-d-old (P21-P25) rats. In P6-P8 rats, induction of LTD depended on the activity of group II mGluRs. In P21-P25 rats, however, this LTD disappeared, and instead, NMDA receptor (NMDAR)-dependent LTD appeared. A bath containing a specific calcineurin (CaN) inhibitor restored the group II mGluR-dependent LTD in the neurons of the P21-P25 rats. Although postsynaptic injection of CaN inhibitors suppressed NMDAR-dependent LTD, it did not affect induction of group II mGluR-dependent LTD. These results demonstrate that CaN plays different roles in the induction of two forms of LTD: presynaptic CaN inhibits group II mGluR-dependent LTD, whereas postsynaptic CaN facilitates NMDAR-dependent LTD. These findings are the first demonstration in vitro of group II mGluR-dependent LTD that is negatively regulated by CaN via an age-dependent mechanism.

MeSH terms

  • Animals
  • Animals, Newborn
  • Calcineurin / physiology*
  • Calcineurin Inhibitors
  • Excitatory Postsynaptic Potentials
  • Hippocampus / cytology
  • Hippocampus / growth & development*
  • Kinetics
  • Models, Neurological
  • Neuronal Plasticity*
  • Presynaptic Terminals / physiology
  • Pyramidal Cells / growth & development*
  • Pyramidal Cells / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Metabotropic Glutamate / antagonists & inhibitors
  • Receptors, Metabotropic Glutamate / physiology*
  • Receptors, N-Methyl-D-Aspartate / physiology*
  • Synapses / physiology

Substances

  • Calcineurin Inhibitors
  • Receptors, Metabotropic Glutamate
  • Receptors, N-Methyl-D-Aspartate
  • metabotropic glutamate receptor 2
  • Calcineurin