Abstract
Genetic studies aimed at eliminating expression of the atp operon (F(0)F(1) H(+)-ATPase) of Streptococcus pneumoniae by genetic disruption of atpC, the first gene of the operon, with a chloramphenicol-resistance cassette were performed. Resistant transformants were obtained only when the recipient strain had a duplication of atpC, recombination occurring in such a way that transcription of the operon from its own promoter was allowed. These results imply that the atp operon is essential for the viability of the cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Membrane / enzymology*
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Chloramphenicol O-Acetyltransferase / genetics
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Chloramphenicol O-Acetyltransferase / metabolism
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Gene Deletion
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Genes, Essential
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Mitochondrial Proton-Translocating ATPases / genetics
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Proton-Translocating ATPases / genetics*
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Proton-Translocating ATPases / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Recombination, Genetic
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Streptococcus pneumoniae / enzymology
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Streptococcus pneumoniae / growth & development*
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Transformation, Bacterial
Substances
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Recombinant Fusion Proteins
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Chloramphenicol O-Acetyltransferase
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mitochondrial ATPase subunit c
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Mitochondrial Proton-Translocating ATPases
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Proton-Translocating ATPases