Detection of aminotransferase activity of Propionibacterium freudenreichii after SDS-PAGE

J Microbiol Methods. 2002 Sep;51(1):57-62. doi: 10.1016/s0167-7012(02)00060-x.

Abstract

Aminotransferases (ATs) had previously been detected after native electrophoresis. We show now that aminotransferase(s) of Propionibacterium freudenreichii can be detected after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Moreover, it retained a high activity (84%) in the presence of 0.23% SDS, contrary to what was observed for aminotransferase(s) of Bifidobacterium bifidum (54%) and of six other cheese-related species (0-20%).

MeSH terms

  • Bifidobacterium / enzymology
  • Electrophoresis, Polyacrylamide Gel
  • Leucine Transaminase
  • Molecular Weight
  • Propionibacterium / enzymology*
  • Transaminases / analysis*
  • Transaminases / metabolism

Substances

  • Transaminases
  • Leucine Transaminase