Abstract
We investigated the effect of estrogens on heart mitochondrial functions and whether estrogens can prevent calcium-induced release of cytochrome c from mitochondria. 10 nM-10 microM 17beta-estradiol or 4-hydroxytamoxifen did not affect mitochondrial respiration rate and membrane potential in state 3 and state 4. Higher concentrations of both agents decreased state 3 respiration rate and membrane potential. 100 nM 17beta-estradiol and 4-hydroxytamoxifen blocked high calcium-induced cytochrome c release from mitochondria but not mitochondrial swelling. Thus, at physiological concentrations estrogens do not affect mitochondrial respiratory functions but protect heart mitochondria from high calcium-induced release of cytochrome c.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium / metabolism
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Calcium / pharmacology
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Cytochrome c Group / metabolism*
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Dose-Response Relationship, Drug
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Estradiol / pharmacology
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Estrogens / metabolism*
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Female
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Ion Channels / metabolism
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Membrane Potentials / drug effects
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Mitochondria, Heart / drug effects
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Mitochondria, Heart / metabolism*
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Mitochondria, Heart / physiology
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Mitochondrial Membrane Transport Proteins
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Mitochondrial Permeability Transition Pore
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Rats
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Rats, Wistar
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Receptors, Estrogen / agonists
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Tamoxifen / analogs & derivatives*
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Tamoxifen / pharmacology
Substances
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Cytochrome c Group
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Estrogens
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Ion Channels
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Mitochondrial Membrane Transport Proteins
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Mitochondrial Permeability Transition Pore
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Receptors, Estrogen
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Tamoxifen
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afimoxifene
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Estradiol
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Calcium